Coding

Part:BBa_K4707000:Design

Designed by: Elise Bennett   Group: iGEM23_UVU-Utah   (2023-09-05)


AMO Coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coding sequence was codon optimized for Chlamydomonas reinhardtii. SapI, BsaI, and BpiI recognition sites were removed where necessary by making silent mutations.


Source

Methylomicrobium album BG8 is the source of this gene. We are then placing this gene into Chlamydomonas reinhardtii for bioremediation of a lake.

References